大鼠肺泡巨噬细胞NR8383
| 【编号】：NR8383 【名称】：大鼠肺泡巨噬细胞NR8383 【规格】：株 【价格】：1800   大鼠肺泡巨噬细胞
细胞名称：NR8383大鼠肺泡巨噬细胞
描述：NR8383 (正常大鼠，1983年8月3日)来源于肺灌洗时的正常大鼠肺泡巨噬细胞。 细胞在gerbil肺细胞连续培养液存在下培养了大约8-9个月。 随后，不再需要外源生长因子。 通过有限稀释法从单个细胞克隆并亚克隆NR8383细胞，并三次用软琼脂亚克隆。 细胞表现出巨噬细胞的特性，吞噬酵母多糖和铜绿，非特异性脂酶活性，Fc受体，氧化降解；分泌IL-1, TNF beta和IL-6，可重复地响应外源生长因子。 NR8383细胞响应博莱霉素，分泌TGF beta前体。 在博莱霉素刺激下，TGF beta mRNA表达也上升。 细胞对内毒素敏感。 1-10 钠克/毫升的LPS水平抑制增生达50%。 即使达到0.001毫克/毫升的水平，LPS抑制还是无毒且在后续过程中可逆的。 NR8383细胞株提供了高响应的肺泡巨噬细胞的均一来源，可以用于体外研究巨响细胞相关活性。
动物种别：大鼠
性别：未知
组织来源：肺；巨噬细胞；肺泡
形态：巨噬细胞
培养基和添加剂：F12K培养基SIGMA,货号N3520，添加NaHCO3 2.5g/L)，80%；优质胎牛血清，20%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
供应限制：A。仅供研究之用。
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