核酸酶S1 来源于米曲霉, ≥100,000-500,000 units/ml
| 【编号】：128634 【产品名称】：核酸酶S1 来源于米曲霉, ≥100,000-500,000 units/ml 【规格】：10KU 【用途】：   核酸酶S1 来源于米曲霉, ≥100,000-500,000 units/ml
Product Name： Nuclease S1 from Aspergillus oryzae
CAS号：37288-25-8
介绍： Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.
用途： Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.
贮存： 储存温度 -20°C
生化和生理学机理： Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.
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| 上一篇：神经氨酸酶 来源于产气荚膜梭菌, ≥0.5 units/mg dry weight | 下一篇：微球菌核酸酶 来源于金黄色葡萄球菌(菌株ATCC #27735), ≥6,000 units/mg pro |
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