白介素10(人类rDNA衍生)WHO参考试剂
【编号】:NIBSC93/722 【名称】:白介素10(人类rDNA衍生)WHO参考试剂 【规格】:个 【价格】:2600元 白介素10(人类rDNA衍生)WHO参考试剂
编号:NIBSC93/722
英文:Interleukin-10 (Human rDNA derived) WHO Reference Reagent
中文:白介素1受体拮抗剂
标准类型:非世卫组织参考材料
规格:1个
描述:1. INTENDED USE
This Standard is the primary biological standard for IL-10.
2. CAUTION
This preparation is not for administration to humans or animals in
the human food chain.
The preparation contains material of human origin, and either the final
product or the source materials, from which it is derived, have been
tested and found negative for HBsAg, anti-HIV and HCV RNA. As with
all materials of biological origin, this preparation should be regarded as
potentially hazardous to health. It should be used and discarded
according to your own laboratory's safety procedures. Such safety
procedures should include the wearing of protective gloves and
avoiding the generation of aerosols. Care should be exercised in
opening ampoules or vials, to avoid cuts.
3. UNITAGE
The assigned potency is 5000 Units of biological activity per ampoule.
4. CONTENTS
Country of origin of biological material: United Kingdom.
Each ampoule contains a freeze dried residue comprising, under an
atmosphere of nitrogen:
IL-10 approximately 1 microgram
9.0 mg sodium chloride
1.0 mg trehalose
2.0 mg human serum albumin
The IL-10 protein was expressed in E. coli.
5. STORAGE
For economy of use, it is recommended that the solution be sub divided
into several small aliquots and stored at -40ºC or below. Avoid repeated
thawing/freezing. Unopened ampoules should be stored at -20ºC
Please note: because of the inherent stability of lyophilized material,
NIBSC may ship these materials at ambient temperature.
6. DIRECTIONS FOR OPENING
DIN ampoules have an 'easy-open' coloured stress point, where the
narrow ampoule stem joins the wider ampoule body. Various types of
ampoule breaker are available commercially. To open the ampoule,
tap the ampoule gently to collect material at the bottom (labelled) end
and follow manufactures instructions provided with the ampoule
breaker.
7. USE OF MATERIAL
No attempt should be made to weigh out any portion of the freeze dried
material. Dissolve the total contents of the ampoule in 0.5ml of sterile
distilled water. Rinse the ampoule with about 0.4ml of sterile distilled
water and make up the total volume to 1.0ml with distilled water. This
solution will contain IL-10 at a concentration of 5000 Units/ml. Use carrier
protein where extensive dilution is required.
8. STABILITY
Reference materials are held at NIBSC within assured, temperaturecontrolled
storage facilities and they should be stored on receipt as
indicated on the label. It is the policy of WHO not to assign an expiry
date to their international reference materials. Accelerated degradation
studies have indicated that this material is suitably stable, when stored at -20ºC or below, for the assigned values to remain valid until the
material is withdrawn or replaced. These studies have also shown that
the material is suitably stable for shipment at ambient temperature
without any effect on the assigned values. Once reconstituted, diluted or
aliquoted, users should determine the stability of the material according
to their own method of preparation, storage and use. Users who have
data supporting any deterioration in the characteristics of any reference
preparation are encouraged to contact NIBSC.
9. REFERENCES
This standard was produced under WHO guidelines cited in the WHO
Technical Reports Series, No.800, 1990, Annex 4.
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